Anti urolithiatic activity of Cyperus rotundus tubers: In silico, In vitro and In vivo approaches

The present research evaluated the anti urolithic potential of Cyperus rotundus tubers extract using in silico, vitro and vivo techniques. In silicostudy was performed constituents pathological protein oxalate oxidase (PDB Id: 2ETE). vitrostudy, nucleation aggregation assay involved for assessment ethanol tuber (50-3000 µg/ml).In studies that rotundusethanolic (100, 200 400 mg/kg B.wt.) wastreatedonsodium induced urolithiatic rats seven days,evaluated kidney function by urine serum biochemical analysis statistical usingGraphPad prism5 software.In silico results showedthat constituents,Humulene epoxide, 4-Oxo-alpha-ylangene, Cubebol were exhibited better binding energyonoxalate oxidase.Ethanolic rotundustuber nucleation, calcium monohydrate crystals inhibition dosedependent manner. Sodium treatment triggered changesin have been substantially prevented ethanolic tuber. current findings activity due to antioxidant essential oils. molecular docking could be used optimize lead develop appropriate urolithiasis treatment.